Prof Arwyn Tomos Jones - BSc (Hons) MSc PhD Dip-Journalism
Member of the School's Drug Delivery & Microbiology Research Discipline
Research projects in his lab fall under themes of cell biology and endocytosis, concentrating on linking fundamental endocytosis research to the remit of cellular delivery of macromolecular therapeutics. Ongoing projects include:
- Characterisation of endocytic pathways utilised by therapeutic macromolecules to gain access into cells
- Studying the mechanism of entry of cell penetrating peptides (CPP) and CPP-conjugates
- Designing new polymer-peptide conjugates for cellular delivery of therapeutic macromolecule
- Utilising subcellular fractionation for analysis of endocytosis and intracellular traffic of therapeutic macromolecules.
- Characterising roles for endocytic pathways in mediating multidrug resistance in cancer cells
- Studying the roles of Rab GTPases in endocytosis
- Design and manufacture of microarray surfaces for multi-image analysis of biological specimens.
Endocytic pathways and the cellular delivery of therapeutic macromolecules
Endocytosis encompasses highly complex and dynamic processes that cells use to allow entry of extracellular material. The utilisation of these pathways for uptake and intracellular delivery of therapeutic macromolecules offers much potential in drug delivery. However, the effectiveness of this approach is constrained by the fact that the fate of the therapeutic within one of these pathways is predetermined by the dynamics of the pathway and biological barriers posed by endocytic membranes. The groups aims are to better understand endocytic pathways of cells, to utilise specific pathways for cellular delivery of therapeutic macromolecules and to design peptides and polymer peptide conjugates for enhancing cytosolic delivery of therapeutics.
Ongoing projects include:
- Pharmacological and molecular manipulation of endocytic pathways to characterise cellular uptake of therapeutic macromolecules
- Characterising the transduction capacities of cell penetrating peptides across biological membranes and their potential as vectors for cellular delivery of therapeutic molecules
- Bioresponsive polymer-peptide conjugates as vectors for cellular delivery of therapeutic macromolecules
- Reorganisation of endocytic pathways in multidrug resistant leukaemia cells.
- Characterising the uptake of targeted nanoparticles against Tuberculosis in macrophages. This is a Bill and Melinda Gates Foundation sponsored collaboration between Cardiff University, the Council for Industrial and Scientific Research South Africa, the University of Pretoria and Nottingham University.
Design and manufacture of microarray surfaces for multi-image analysis of biological specimens.
Imaging whole cells and cellular components is now routine practice, and this is due in part to major advances in electron and fluorescence microscopy. Alone, sub disciplines of each of these techniques provide information regarding the dynamics of live systems (fluorescence microscopy) and then ultrastructural information is gained by electron microscopy. The aims of this project are to manufacture grid-based surfaces that allow multi-image analysis on single specimens.