MOLECULAR BIOLOGY

DNA ANALYSIS

Agarose gel electrophoresis

Measuring DNA concentration and purity

Estimating concentrations of DNA on an agarose gel

Automated sequencing of purified DNA with infrared labelled primers and thermo-sequenase

Automated sequencing of purified DNA by inclusion of fluorescent dideoxy nucleotides

BACTERIAL CELL TRANSFORMATION

Pouring agar plates with antibiotic

Competent cell preparation [CaCl2]

Transformation [CaCl2]

PLASMID DNA PREPARATION

Mini-preparation of DNA Promega SV

Mini-preparation of DNA (Sequencing Grade)

Mini-Preparation of DNA - Phenol Chloroform extraction

DNA MODIFICATION

Restriction endonuclease digestion of DNA

Ligations

Dephosphorylation

Concentration / precipitation of nucleic acids

Removal of protein contamination from nucleic acids

DNA PURIFICATION

Tip gel extraction method

Hybaid gel extraction of DNA

QIAquick Gel Extraction (QIAGEN)

RNA PURIFICATION AND MODIFICATION

mRNA extraction (Pharmacia)

Pharmacia QuickPrep Micro mRNA Purification Kit

Reverse transcriptase reaction

In vitro translation

POLYMERASE CHAIN REACTION

Standard polymerase chain reaction

Direct sequencing of PCR products

RACE

ARRAYS

Differential Southern (PCR Screen of Subtracted Gene Array)

CLONING STRATEGIES