The Art of Gene Expression in E. coli

Maltose transporter
DNA sequences  
Dana's tools  

Gene expression in
E. coli

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© 2001 Webdesign by
Tilman Maier og Lars Jessen
1. Factors that influence Gene Expression
2. Expression vectors
3. Folding
4. Translocation
5. Protein stability
6. How to address the hopeless case
7. Safety
8. Links

7. Safety

Use plasmid vectors that
  1. do not replicate in human cells
  2. do not express genes in human cells
  3. do not contain antibiotic resistance markers
  4. do not even replicate in other bacterial cells (e.g. R6K vectors require the pir gene)
Use E. coli cells that
  1. do not contain F' factors
  2. do not contain prophages (this will be hard to achieve)