The Art of Gene Expression in E. coli



Databases
ECCE   
Maltose transporter
DNA sequences  
Software
Dana's tools  
Strategy

Gene expression in
E. coli




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Tilman Maier og Lars Jessen
1. Factors that influence Gene Expression
2. Expression vectors
3. Folding
4. Translocation
5. Protein stability
6. How to address the hopeless case
7. Safety
8. Links

7. Safety

Use plasmid vectors that
  1. do not replicate in human cells
  2. do not express genes in human cells
  3. do not contain antibiotic resistance markers
  4. do not even replicate in other bacterial cells (e.g. R6K vectors require the pir gene)
Use E. coli cells that
  1. do not contain F' factors
  2. do not contain prophages (this will be hard to achieve)