The Art of Gene Expression in E. coli
Databases
ECCE
Maltose transporter
DNA sequences
Software
Dana's tools
Strategy
Gene expression in
E. coli
Search all files of this site
© 2001 Webdesign by
Tilman Maier
og
Lars Jessen
1. Factors that influence Gene Expression
2. Expression vectors
3. Folding
4. Translocation
5. Protein stability
6. How to address the hopeless case
7. Safety
8. Links
7. Safety
Use plasmid vectors that
do not replicate in human cells
do not express genes in human cells
do not contain antibiotic resistance markers
do not even replicate in other bacterial cells (e.g. R6K vectors require the pir gene)
Use E. coli cells that
do not contain F' factors
do not contain prophages (this will be hard to achieve)