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Gene expression in
E. coli


TEV NIa protease
as a tool




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© 2001 Webdesign by
Tilman Maier og Lars Jessen
Some guidelines for successful gene expression in E. coli including an overview of the many factors that can influence gene expression such as codon usage, expression vectors, choice of cellular compartment, translocation and folding competence, proteases, and manipulation of translation efficiency.

1. Factors that influence Gene Expression
2. Expression vectors
3. Folding
4. Translocation
5. Protein stability
6. How to address the hopeless case
7. Safety
8. Links