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ecce/ outer membrane proteins/ enzymes
Hydrolases
-acting on ester bonds
Carboxylic ester hydrolases
Phosphoric monoester hydrolases
-Glucosidases:
hydrolysing O-glycosyl compounds
-acting on peptide Bonds:
Serine endopeptidase

Hydrolases Acting on Ester Bonds

 Carboxylic Ester Hydrolases


Phospholipase A1 precursor (pldA )
functionhydrolysis of phosphatidylcholine with phospholipase A2 and phospholipase A1 activities. Required for efficient secretion of bacteriocins, seems to be dormant in normal growing cells
interaction 
cofactorsrequires calcium ions for activity
regulationinduction: by membrane damaging, e.g. phage-induced lysis or temperature shock
structure3d structurePDB
MW33163; 289 aa


Phosphoric Monoester Hydrolases

Phosphatidylglycerophosphatase B (pgpB )

functionhydrolyzes phosphatidylglycerophosphate, phosphatidic acid and lysophosphatic acid, the pattern of activities varies according to subcellular location
interaction 
cofactors 
regulation 
 integral membrane protein of the inner and the outer membrane
MW29021; 254 aa


Glucosidases Hydrolysing O-Glycosyl Compounds:

Membrane-bound lytic murein Transglycosylase A precursor (mltA )
functionmurein-degrading enzyme, may play a role in recycling of muropeptides during cell elongation and/or cell division
interaction 
cofactors 
regulationoptimal activity is between pH 4.0 and 4.5, loses its activity rapidly above 30°C
 attached to the outer membrane by a lipid anchor (probable)
MW40410; 365 aa


Membrane-bound lytic murein Transglycosylase B precursor (mltB )
functionmurein-degrading enzyme, may play a role in recycling of muropeptides during cell elongation and/or cell division
interaction 
cofactors 
regulation 
 attached to the outer membrane by a lipid anchor and exposed to the periplasmic side (probable)
structure3d structurePDB
MW40256; 361 aa


Membrane-bound lytic murein Transglycosylase C precursor (mltC )
functionmurein-degrading enzyme, may play a role in recycling of muropeptides during cell elongation and/or cell division
interaction 
cofactors 
regulation 
 attached to the outer membrane by a lipid anchor (probable)
MW40112; 359 aa


Membrane-bound lytic murein Transglycosylase D precursor (mltD )
functionmurein-degrading enzyme, may play a role in recycling of muropeptides during cell elongation and/or cell division
interaction 
cofactors 
regulationoptimal activity is between pH 4.0 and 4.5, loses its activity rapidly above 30°C
 attached to the outer membrane by a lipid anchor (probable)

3d structurePDB

MW49417; 452 aa


Membrane-bound lytic murein Transglycosylase E precursor (mltE )
functionmurein-degrading enzyme, may play a role in recycling of muropeptides during cell elongation and/or cell division
interaction 
cofactors 
regulation 
MW26574; 241 aa


Hydrolases acting on peptide Bonds

Serine Endopeptidases


Outer membrane protease OmpP precursor (ompP )
functionprotease, also acts as receptor for bacteriophage Ox2
interaction 
cofactors 
regulation 
 homopentamer
MW35499; 315 aa


Protease VII Precursor (ompT )
functioncleaves T7 RNA polymerase, ferric enterobactin receptor protein and other proteins, specificity for paired basic residues
The 3D structure questions that OmpT is a ser protease.
interaction 
cofactors 
regulationinhibited by zinc ions
structurePDB
MW35562; 317 aa