Bacillus subtilis Stock Collection

Strain	MD157	Synonymous	WH352	BGSC	N^o
Genotype		trpC2 amyE::(spoVG-lacZ, Cm)
Construction		Tf. plasmid pDH32M in B. subtilis 168
Special phenotypes		AmyE minus phenotype on LB starch plates
Date of conctruction		05.02.1994
Source / Investigator		M.K.Dahl
Published in		Dahl, M.K., J. Degenkolb and W. Hillen. 1994. Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs. J. Mol. Biol. 242:413-427.

Strain	MD185	Synonymous		BGSC	N^o
Genotype		treC2 glcK::aphA3
Construction		Tf of plasmid pMD494 in B. subtilis 168 selected on LB Km(30)
Special phenotypes		Glucose kinase minus
Date of conctruction		20.09.1996
Source / Investigator		Pierre Skarlatos
Published in		Skarlatos, P. and M.K. Dahl. 1998. The glucose kinase of Bacillus subtilis. J. Bacteriol. 180(12):3222-3226.

Strain	MD186	Synonymous		BGSC	N^o
Genotype		trpC2 glcK::spe
Construction		Tf. of plasmid pMD495 in B. subtilis 168 selected on LB Sp(100)
Special phenotypes		Glucose kinase minus, tested in enzyme assay
Date of conctruction		06.01.1997
Source / Investigator		Pierre Skarlatos
Published in		Skarlatos, P. and M.K. Dahl. 1998. The glucose kinase of Bacillus subtilis. J. Bacteriol. 180(12):3222-3226.

Strain	MD192	Synonymous		BGSC	N^o
Genotype		trpC2 malL::aphA3
Construction		Integration of plasmid pmalLK2 by double cross over, selected on kanamycin, screen by PCR
Special phenotypes		MalL minus aphA3 cassette in opossite orientation of the operon
Date of conctruction		11.11.1997
Source / Investigator		Stefan Schönert
Published in		Schönert S., T. Buder, and M.K. Dahl. 1998. Identification and enzymatic characterization of the maltose-inducible a-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis. J. Bacterol. 180(9):2574-2578.

Strain	MD193	Synonymous		BGSC	N^o
Genotype		trpC2 malL::aphA3
Construction		Integration of plasmid pmalLK1 by double cross over, selected on kanamycin, screen by PCR
Special phenotypes		MalL minus aphA3 in orientation of the operon
Date of conctruction		11.11.1997
Source / Investigator		Stefan Schönert
Published in		Schönert S., T. Buder, and M.K. Dahl. 1998. Identification and enzymatic characterization of the maltose-inducible a-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis. J. Bacterol. 180(9):2574-2578.

Strain	MD215	Synonymous		BGSC	N^o
Genotype		trpC2 malE::aphA3
Construction		Tf. of pMBPK1-Sac I+Fsp I into B. subtilis 168, selection on kanamycin 30 µg / ml. screen by PCR and western blot analysis
Special phenotypes		aphA3 in same orientation of the malE gene
Date of conctruction		16.11.1998
Source / Investigator		Stefan Schönert
Published in

Strain	MD216	Synonymous		BGSC	N^o
Genotype		trpC2 malE::aphA3
Construction		Tf. of pMBPK2-Sac I+Fsp I into B. subtilis 168, selection on kanamycin 30 µg / ml. screen by PCR and western blot analysis
Special phenotypes		aphA3 in the opposite direction of the malE gene
Date of conctruction		16.11.1998
Source / Investigator		Stefan Schönert
Published in

Strain	MD217	Synonymous		BGSC	N^o
Genotype		trpC2 pgcM::aphA3
Construction		Tf. of pLRßM1-Km1 into B. subtilis 168, selection on kanamycin 30 µg / ml.
Special phenotypes		aphA3 in same orientation of the pgcM gene
Date of conctruction		23.06.1999
Source / Investigator		Lili Rosana Mesak
Published in

Strain	MD218	Synonymous		BGSC	N^o
Genotype		trpC2 pgcM::aphA3
Construction		Tf. of pLRßM1-Km2 into B. subtilis 168, selection on kanamycin 30 µg / ml
Special phenotypes		aphA3 in the opposite direction of the pgcM gene
Date of conctruction		23.06.1999
Source / Investigator		Lili Rosana Mesak
Published in

Strain	MD219	Synonymous		BGSC	N^o
Genotype		trpC2 malE::aphA3 glvC::spc
Construction		Tf. of chr. B.subtilis GP110 (glvC^-) into B. subtilis MD215, selection on kanamycin 5 µg / ml and spectinomycine 100 µg / ml
Special phenotypes		aphA3 in same orientation of the malE gene
Date of conctruction		23.06.1999
Source / Investigator		Stefan Schönert
Published in

Strain	MD220	Synonymous		BGSC	N^o
Genotype		trpC2 malE::aphA3 glvC::spc
Construction		Tf. of chr. B.subtilis GP110 (glvC^-) into B. subtilis MD216, selection on kanamycin 5 µg / ml and spectinomycine 100 µg / ml
Special phenotypes		aphA3 in the opposite direction of the malE gene
Date of conctruction		26.06.1999
Source / Investigator		Stefan Schönert
Published in

Strain	MD221	Synonymous		BGSC	N^o
Genotype		trpC2 malE::aphA3 amyE::cm
Construction		Tf. of chr. B.subtilis MD215 (malE ^- ) in B.subtilis MD195, selection on Km 30 µg / ml and Cm 5 µg / ml.
Special phenotypes		aphA3 in the same direction of the malE gene, AmyE minus
Date of conctruction		16.02.2000
Source / Investigator		Stefan Schönert
Published in

Strain	MD222	Synonymous		BGSC	N^o
Genotype		trpC2 malE::aphA3 amyE::cm
Construction		Tf. of chr. B.subtilis MD216 (malE ^- ) in B.subtilis MD195, selection on Km 25 µg / ml and Cm 5 µg / ml.
Special phenotypes		aphA3 in the opposite direction of the malE gene
Date of conctruction		16.02.2000
Source / Investigator		Stefan Schönert
Published in

Strain	MD223	Synonymous		BGSC	N^o
Genotype		trpC2 malR::aphA3
Construction		Tf of pMalRK1 (malR ^- ) in B. subtilis 168, selection on Km 25 µg / ml.
Special phenotypes		aphA3 in the same direction of the malR gene
Date of conctruction		16.02.2000
Source / Investigator		Stefan Schönert
Published in

Strain	MD224	Synonymous		BGSC	N^o
Genotype		trpC2 malR::aphA3
Construction		Tf. of pMalRK2 (malR - ) in B. subtilis 168, selection on Km 25 µg / ml.
Special phenotypes		aphA3 in the opposite direction of the malR gene
Date of conctruction		16.02.2000
Source / Investigator		Stefan Schönert
Published in

Strain	MD225	Synonymous		BGSC	N^o
Genotype		trpC2 yvdF::aphA3
Construction		Tf. of pYvdFK1 (yvdF::aphA3 ) in B. subtilis 168, selection on Km 25 µg / ml.
Special phenotypes		aphA3 in the same direction of the yvdF gene
Date of conctruction		16.02.2000
Source / Investigator		Sabine Kastner
Published in

Strain	MD226	Synonymous		BGSC	N^o
Genotype		trpC2 yvdF::aphA3
Construction		Tf. of pYvdFK2 (yvdF::aphA3 ) in B. subtilis 168, selection on Km 25 µg / ml.
Special phenotypes		aphA3 in the opposite direction of the yvdF gene
Date of conctruction		16.02.2000
Source / Investigator		Sabine Kastner
Published in

Strain	MD227	Synonymous		BGSC	N^o
Genotype		trpC2 yvdF::aphA3 amyE::Cm
Construction		Tf. of chr. B.subtilis MD225 (yvdF - ) in B.subtilis MD195, selection on Km 25 µg / ml and Cm 5 µg / ml.
Special phenotypes		aphA3 in the same direction of the yvdF gene
Date of conctruction		16.02.2000
Source / Investigator		Sabine Kastner
Published in

Strain	MD228	Synonymous		BGSC	N^o
Genotype		trpC2 yvdF::aphA3 amyE::Cm
Construction		Tf. of chr. B.subtilis MD226 (yvdF - ) in B.subtilis MD195, selection on Km 25 µg / ml and Cm 5 µg / ml.
Special phenotypes		aphA3 in the opposite direction of the yvdF gene
Date of conctruction		16.02.2000
Source / Investigator		Sabine Kastner
Published in