Strain

MD157

Synonymous

WH352

BGSC

No

 

Genotype

trpC2 amyE::(spoVG-lacZ, Cm)

Construction

Tf. plasmid pDH32M in B. subtilis 168

Special phenotypes

AmyE minus phenotype on LB starch plates

Date of conctruction

05.02.1994

Source / Investigator

M.K.Dahl

Published in

Dahl, M.K., J. Degenkolb and W. Hillen. 1994. Transcription of the xyl operon is controlled in Bacillus subtilis by tandem overlapping operators spaced by four base-pairs. J. Mol. Biol. 242:413-427.

 

Strain

MD185

Synonymous

 

BGSC

No

 

Genotype

treC2 glcK::aphA3

Construction

Tf of plasmid pMD494 in B. subtilis 168 selected on LB Km(30)

Special phenotypes

Glucose kinase minus

Date of conctruction

20.09.1996

Source / Investigator

Pierre Skarlatos

Published in

Skarlatos, P. and M.K. Dahl. 1998. The glucose kinase of Bacillus subtilis. J. Bacteriol. 180(12):3222-3226.

 

Strain

MD186

Synonymous

 

BGSC

No

 

Genotype

trpC2 glcK::spe

Construction

Tf. of plasmid pMD495 in B. subtilis 168 selected on LB Sp(100)

Special phenotypes

Glucose kinase minus, tested in enzyme assay

Date of conctruction

06.01.1997

Source / Investigator

Pierre Skarlatos

Published in

Skarlatos, P. and M.K. Dahl. 1998. The glucose kinase of Bacillus subtilis. J. Bacteriol. 180(12):3222-3226.

 

Strain

MD192

Synonymous

 

BGSC

No

 

Genotype

trpC2 malL::aphA3

Construction

Integration of plasmid pmalLK2 by double cross over, selected on kanamycin, screen by PCR

Special phenotypes

MalL minus aphA3 cassette in opossite orientation of the operon

Date of conctruction

11.11.1997

Source / Investigator

Stefan Schönert

Published in

Schönert S., T. Buder, and M.K. Dahl. 1998. Identification and enzymatic characterization of the maltose-inducible a-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis. J. Bacterol. 180(9):2574-2578.

 

Strain

MD193

Synonymous

 

BGSC

No

 

Genotype

trpC2 malL::aphA3

Construction

Integration of plasmid pmalLK1 by double cross over, selected on kanamycin, screen by PCR

Special phenotypes

MalL minus

aphA3 in orientation of the operon

Date of conctruction

11.11.1997

Source / Investigator

Stefan Schönert

Published in

Schönert S., T. Buder, and M.K. Dahl. 1998. Identification and enzymatic characterization of the maltose-inducible a-glucosidase MalL (sucrase-isomaltase-maltase) of Bacillus subtilis. J. Bacterol. 180(9):2574-2578.

 

Strain

MD215

Synonymous

 

BGSC

No

 

Genotype

trpC2 malE::aphA3

Construction

Tf. of pMBPK1-Sac I+Fsp I into B. subtilis 168, selection on kanamycin 30 g / ml. screen by PCR and western blot analysis

Special phenotypes

aphA3 in same orientation of the malE gene

Date of conctruction

16.11.1998

Source / Investigator

Stefan Schönert

Published in

 

Strain

MD216

Synonymous

 

BGSC

No

 

Genotype

trpC2 malE::aphA3

Construction

Tf. of pMBPK2-Sac I+Fsp I into B. subtilis 168, selection on kanamycin 30 g / ml. screen by PCR and western blot analysis

Special phenotypes

aphA3 in the opposite direction of the malE gene

Date of conctruction

16.11.1998

Source / Investigator

Stefan Schönert

Published in

 

Strain

MD217

Synonymous

 

BGSC

No

 

Genotype

trpC2 pgcM::aphA3

Construction

Tf. of pLRßM1-Km1 into B. subtilis 168, selection on kanamycin 30 g / ml.

Special phenotypes

aphA3 in same orientation of the pgcM gene

Date of conctruction

23.06.1999

Source / Investigator

Lili Rosana Mesak

Published in

 

Strain

MD218

Synonymous

 

BGSC

No

 

Genotype

trpC2 pgcM::aphA3

Construction

Tf. of pLRßM1-Km2 into B. subtilis 168, selection on kanamycin 30 g / ml

Special phenotypes

aphA3 in the opposite direction of the pgcM gene

Date of conctruction

23.06.1999

Source / Investigator

Lili Rosana Mesak

Published in

 

Strain

MD219

Synonymous

 

BGSC

No

 

Genotype

trpC2 malE::aphA3 glvC::spc

Construction

Tf. of chr. B.subtilis GP110 (glvC-) into B. subtilis MD215, selection on kanamycin 5 g / ml and spectinomycine 100 g / ml

Special phenotypes

aphA3 in same orientation of the malE gene

Date of conctruction

23.06.1999

Source / Investigator

Stefan Schönert

Published in

 

Strain

MD220

Synonymous

 

BGSC

No

 

Genotype

trpC2 malE::aphA3 glvC::spc

Construction

Tf. of chr. B.subtilis GP110 (glvC-) into B. subtilis MD216, selection on kanamycin 5 g / ml and spectinomycine 100 g / ml

Special phenotypes

aphA3 in the opposite direction of the malE gene

Date of conctruction

26.06.1999

Source / Investigator

Stefan Schönert

Published in

 

Strain

MD221

Synonymous

 

BGSC

No

 

Genotype

trpC2 malE::aphA3 amyE::cm

Construction

Tf. of chr. B.subtilis MD215 (malE - ) in B.subtilis MD195, selection on Km 30 g / ml and Cm 5 g / ml.

Special phenotypes

aphA3 in the same direction of the malE gene, AmyE minus

Date of conctruction

16.02.2000

Source / Investigator

Stefan Schönert

Published in

 

 

 

 

 

 

 

 

 

Strain

MD222

Synonymous

 

BGSC

No

 

Genotype

trpC2 malE::aphA3 amyE::cm

Construction

Tf. of chr. B.subtilis MD216 (malE - ) in B.subtilis MD195, selection on Km 25 g / ml and Cm 5 g / ml.

Special phenotypes

aphA3 in the opposite direction of the malE gene

Date of conctruction

16.02.2000

Source / Investigator

Stefan Schönert

Published in

 

Strain

MD223

Synonymous

 

BGSC

No

 

Genotype

trpC2 malR::aphA3

Construction

Tf of pMalRK1 (malR - ) in B. subtilis 168, selection on Km 25 g / ml.

Special phenotypes

aphA3 in the same direction of the malR gene

Date of conctruction

16.02.2000

Source / Investigator

Stefan Schönert

Published in

 

Strain

MD224

Synonymous

 

BGSC

No

 

Genotype

trpC2 malR::aphA3

Construction

Tf. of pMalRK2 (malR - ) in B. subtilis 168, selection on Km 25 g / ml.

Special phenotypes

aphA3 in the opposite direction of the malR gene

Date of conctruction

16.02.2000

Source / Investigator

Stefan Schönert

Published in

 

Strain

MD225

Synonymous

 

BGSC

No

 

Genotype

trpC2 yvdF::aphA3

Construction

Tf. of pYvdFK1 (yvdF::aphA3 ) in B. subtilis 168, selection on Km 25 g / ml.

Special phenotypes

aphA3 in the same direction of the yvdF gene

Date of conctruction

16.02.2000

Source / Investigator

Sabine Kastner

Published in

 

 

 

 

 

Strain

MD226

Synonymous

 

BGSC

No

 

Genotype

trpC2 yvdF::aphA3

Construction

Tf. of pYvdFK2 (yvdF::aphA3 ) in B. subtilis 168, selection on Km 25 g / ml.

Special phenotypes

aphA3 in the opposite direction of the yvdF gene

Date of conctruction

16.02.2000

Source / Investigator

Sabine Kastner

Published in

 

Strain

MD227

Synonymous

 

BGSC

No

 

Genotype

trpC2 yvdF::aphA3 amyE::Cm

Construction

Tf. of chr. B.subtilis MD225 (yvdF - ) in B.subtilis MD195, selection on Km 25 g / ml and Cm 5 g / ml.

Special phenotypes

aphA3 in the same direction of the yvdF gene

Date of conctruction

16.02.2000

Source / Investigator

Sabine Kastner

Published in

 

Strain

MD228

Synonymous

 

BGSC

No

 

Genotype

trpC2 yvdF::aphA3 amyE::Cm

Construction

Tf. of chr. B.subtilis MD226 (yvdF - ) in B.subtilis MD195, selection on Km 25 g / ml and Cm 5 g / ml.

Special phenotypes

aphA3 in the opposite direction of the yvdF gene

Date of conctruction

16.02.2000

Source / Investigator

Sabine Kastner

Published in