TEV NIa protease as a tool for in vitro and in vivo proteolysis



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Gene expression in
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TEV NIa protease
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© 2001 Webdesign by
Tilman Maier og Lars Jessen
1. Introducing a TEV protease recognition site
2. Expression vectors
3. Purification and storage of TEV protease
4. Proteolysis in vitro
5. Proteolysis of whole cells
6. Proteolysis in vivo
7. References
8. Links

6. Proteolysis in vivo

  • Coexpress plasmids encoding target protein and TEV protease
    Do a titration experiment with various inducer concentrations for TEV protease plasmid
    Try various growth conditions (temperatures, media etc)
    Determine the kinetics of cleavage in a pulse chase experiment.