TEV NIa protease as a tool for in vitro and in vivo proteolysis

Maltose transporter
DNA sequences  
Dana's tools  

Gene expression in
E. coli

TEV NIa protease
as a tool

  Search all files of this site

© 2001 Webdesign by
Tilman Maier og Lars Jessen
1. Introducing a TEV protease recognition site
2. Expression vectors
3. Purification and storage of TEV protease
4. Proteolysis in vitro
5. Proteolysis of whole cells
6. Proteolysis in vivo
7. References
8. Links

6. Proteolysis in vivo

  • Coexpress plasmids encoding target protein and TEV protease
    Do a titration experiment with various inducer concentrations for TEV protease plasmid
    Try various growth conditions (temperatures, media etc)
    Determine the kinetics of cleavage in a pulse chase experiment.