Protein

Gene

Synonyme

purified

antibodies

knock out mutants

functional analysis

Function

Reference /

Investigator

MalR

malR

yvdE

yes

 

constructed

in progress

putative regulator

Schönert & Dahl

MalN

malN

yvdF

yes

 

constructed

in progress

putative neopullulanase

Kastner, Schönert & Dahl

MalE

malE

yvdG

yes

yes

constructed

surface plasmon resonance, fluorescence spectroscopy, circular dicroism

Maltodextrin-binding protein;

low affinity to maltose

Schönert, Kastner, Schubert & Dahl, in prep.

MalL

malL

yvdL

yes

 

constructed

enzymatically

a-1,4-1,6-glucosidase

Schönert et al., 1998; 1999

YvdK

yvdK

 

yes

 

constructed

 

unknown

Seitz, Yang & Dahl

YvfK

yvfK

 

yes

yes

constructed

surface plasmon resonance, fluorescence spectroscopy, circular dicroism

Cyclodextrin-binding protein

Kamionka & Dahl, in prep.

PgcM

pgcM

yvdM

yes

 

constructed

enzymatically, fluorescence spectroscopy

ß-phosphoglucomutase /

glucose-1-P-Phosphodismutase

Rosana & Dahl, submitted

GlcK

glcK

yqgR

yes

 

constructed

emzymatically, fluorescence spectroscopy, surface plasmon resonance, circular dicroism

Glucose kinase

Skarlatos & Dahl, 1998, Rosana et al., 1999; Rosana et al., in prep.

GlvA

glvA

 

yes

   

enzymatically

NAD-dependent phospho-a-1,4-glucosidase

Thompson et al., 1998

GlvC

glvC

 

no

 

constructed

knock out mutant; maltose transport assay; determination of generation time on maltose

Enzyme II BC of maltose PTS

Thompson et al., 1998; Reizer et al., 1999, Seitz et al., in prep.